Histochemical mapping of NADPH-diaphorase in monkey and human eyes.

Kaufman Lab // Publications // Apr 30 1998

PubMed ID: 9561828

Author(s): Chen Z, Gu Q, Kaufman PL, Cynader MS. Histochemical mapping of NADPH-diaphorase in monkey and human eyes. Curr Eye Res. 1998 Apr;17(4):370-9.

Journal: Current Eye Research, Volume 17, Issue 4, Apr 1998

PURPOSE To localize nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) in normal human and monkey eyes.

METHODS NADPH-d activity in ocular tissue sections was revealed by histochemical staining.

RESULTS NADPH-d staining was found in the anterior and posterior ocular segment of both species. In the anterior segment, reaction product was present in the corneal epithelium and endothelium, iris dilator and sphincter, lens capsule, ciliary muscle, and non-pigmented epithelial cells and stroma of the ciliary processes. Staining density in the longitudinal ciliary muscle was higher than in the other ciliary muscle regions in the human; such regionalization was less apparent in the monkey. The trabecular meshwork stained minimally if at all in both species. In the posterior segment, staining was seen around choroidal blood vessels and choroidal nerves, and in the retinal pigment epithelial, photoreceptor, bipolar, inner plexiform and ganglion cell layers.

CONCLUSIONS NADPH-d is widespread in human and monkey eyes, indicating significant and presumably functionally relevant NO production in the various ocular structures.