Development and validation of methods to visualize conventional aqueous outflow pathways in canine primary angle closure glaucoma.

PubMed ID: 34581493

Author(s): Telle MR, Snyder KC, Oikawa K, Nilles JP, Gehrke S, Teixeira LBC, Kiland JA, Huang A, McLellan GJ. Development and validation of methods to visualize conventional aqueous outflow pathways in canine primary angle closure glaucoma. Vet Ophthalmol. 2021 Sep 28. doi: 10.1111/vop.12943. [Epub ahead of print] PMID 34581493

Journal: Veterinary Ophthalmology, Sep 2021

PURPOSE Angle closure glaucoma (PACG) is highly prevalent in dogs and is often refractory to medical therapy. We hypothesized that pathology affecting the post-trabecular conventional aqueous outflow pathway contributes to persistent intraocular pressure (IOP) elevation in dogs with PACG. The goal of this study was to determine the potential for aqueous angiography (AA) and optical coherence tomography (OCT) to identify abnormalities in post-trabecular aqueous outflow pathways in canine PACG.

METHODS AA and anterior segment OCT (Spectralis HRA + OCT) were performed ex vivo in 19 enucleated canine eyes (10 normal eyes and 9 irreversibly blind eyes from canine patients enucleated for management of refractory PACG). Eyes were cannulated and maintained at physiologic IOP (10-20 mmHg) prior to intracameral infusion of fluorescent tracer. OCT scleral line scans were acquired in regions of high and low perilimbal AA signal. Eyes were then perfusion fixed and cryosections prepared from 10/10 normal and 7/9 PACG eyes and immunolabeled for a vascular endothelial marker.

RESULTS Normal canine eyes showed segmental, circumferential limbal AA signal, whereas PACG eyes showed minimal or no AA signal. AA signal correlated with scleral lumens on OCT in normal dogs, but lumens were generally absent or flattened in PACG eyes. Collapsed vascular profiles were identified in tissue sections from PACG eyes, including those in which no lumens were identified on AA and OCT.

CONCLUSIONS In canine eyes with PACG, distal aqueous outflow channels are not identifiable by AA, despite normalization of their IOP, and intra-scleral vascular profiles are collapsed on OCT and histopathology.