PubMed ID: 37655900
Author(s): Liu C, Yuan W, Yang H, Ni J, Tang L, Zhao H, Neumann D, Ding X, Zhu L. Associating bovine herpesvirus 1 envelope glycoprotein gD with activated phospho-PLC-gamma1(S1248). Microbiol Spectr. 2023 Oct 17;11(5):e0196323. doi: 10.1128/spectrum.01963-23. Epub 2023 Sep 1. PMID 37655900
Journal: Microbiology Spectrum, Volume 11, Issue 5, Oct 2023
Bovine herpesvirus 1 (BoHV-1) productive infection increases protein levels of phosphorylated-phospholipase C gamma 1 at Ser1248 [p-PLC-γ1(S1248)]. However, whether it causes any variations to p-PLC-γ1(S1248) localization is not well understood. Here, for the first time, we found that partial p-PLC-γ1(S1248) is residing in the Golgi apparatus, where the accumulation is enhanced by virus infection. p-PLC-γ1(S1248) is consistently associated with virions, partially via binding to gD, in both the Golgi apparatus and cytoplasm membranes. Surprisingly, it also associates with the released virions. Of note, this is the first evidenced BoHV-1 virion-bound host protein. It seems that p-PLC-γ1(S1248) works as an escort during trafficking of progeny virions out of Golgi apparatus to the plasma membranes as well as releasing outside of the cell membranes. Furthermore, we showed that the activated p-PLC-γ1(S1248) is potentially implicated in the transport of virions out of Golgi apparatus, which may represent a novel mechanism to regulate virus productive infection.