In vitro maintenance of a pure-cone retina.

Nansi Colley // Publications // May 01 1986

PubMed ID: 3516917

Author(s): Williams DS, Colley NJ, Anderson DH, Farber DB, Fisher SK. In vitro maintenance of a pure-cone retina. Invest Ophthalmol Vis Sci. 1986 May;27(5):666-73.

Journal: Investigative Ophthalmology & Visual Science, Volume 27, Issue 5, May 1986

The retina of the lizard, Sceloporus occidentalis, appears to have only cone photoreceptors. Eyecups from this animal were incubated in media containing Earle’s Balanced Salts, supplemented with amino acids and vitamins, and gassed with 5% CO2/95% O2. Under these conditions, good morphology, protein synthesis, and normal cyclic AMP and cyclic GMP levels were maintained for 1-2 days. This in vitro preparation is likely to be useful for pharmacological studies of cone photoreceptors.