Plasmid mediated mutagenesis of a cellular gene in transfected eukaryotic cells.

Brandt Lab // Publications // Jan 26 1987

PubMed ID: 3469621

Author(s): Brandt CR, Buonaguro FM, McDougall JK, Galloway DA. Plasmid mediated mutagenesis of a cellular gene in transfected eukaryotic cells. Nucleic Acids Res. 1987 Jan 26;15(2):561-73.

Journal: Nucleic Acids Research, Volume 15, Issue 2, Jan 1987

NIH3T3 cells are widely used in transformation assays and readily take up transfected DNA. A system has been devised using NIH3T3 cells to measure the mutagenic effect of transfected DNA on recipient cell genes. NIH3T3 cells can be mutated to 6-thioguanine resistance at a frequency which suggests that at least a portion of the cells have only one functional copy of the HGPRT gene. They have a low spontaneous background mutation frequency (approximately 1 X 10(-7)). Transfection of three different plasmids into NIH3T3 cells induced 6-thioguanine resistant mutants at frequencies ranging from 3 to 11 fold above background. The mutant phenotype is stable and reversion frequencies of several mutants are less than or equal to 1 X 10(-7). Southern blot analysis of the HGPRT gene in several mutants showed that 4 of 26 mutants (15.4%) had detectable alterations in the structure of the HGPRT gene. Interestingly 3 of the 4 mutants showing rearrangements were obtained by transfection of the HSV-2 morphological transforming region.