Late-phase reaction in topically induced ocular anaphylaxis in the rat.

Neal Barney // Publications // May 01 1988

PubMed ID: 3136973

Author(s): Trocmé SD, Bonini S, Barney NP, Bloch KJ, Allansmith MR. Late-phase reaction in topically induced ocular anaphylaxis in the rat. Curr Eye Res. 1988 May;7(5):437-43. PMID 3136973

Journal: Current Eye Research, Volume 7, Issue 5, May 1988

A cellular late-phase reaction is described in a rat model of topically induced ocular anaphylaxis. Rats were immunized with dinitrophenylated Ascaris suum extract and alum and were tested for active cutaneous anaphylaxis on day 13. Rats with a strong skin test response were selected for ocular challenge with di-DNP-lysine. Macroscopic observation and histologic evaluation were performed at 1, 6, and 24 h. In rats showing a moderate macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 h; no significant increase in eosinophils, neutrophils or lymphocytes was found in the conjunctive of these animals. In rats showing a marked macroscopic ocular response at 1 h, mast cell degranulation was significantly increased at 1 and 6 h; the number of eosinophils was significantly increased at 1 and 6 h, and of neutrophils at 6 h only. At 24 h, neutrophil and eosinophil numbers returned to baseline levels. There was no macroscopic evidence of a late-phase response in either group of animals. Our results suggest that, in keeping with earlier observations in human skin, a strong early response to antigen is required for the development of a late-phase ocular response in the rat.