Author(s):Griep AE, Westphal H. Antisense Myc sequences induce differentiation of F9 cells. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6806-10. PMID 2842791
Journal: Proceedings Of The National Academy Of Sciences Of The United States Of America, Volume 85, Issue 18, Sep 1988
Down-regulation of Myc expression is the earliest documented change in gene expression in retinoic acid-induced differentiation of murine F9 teratocarcinoma cells. F9 cells transfected with plasmids expressing antisense Myc sequences under control of the simian virus 40 (SV40) early promoter exhibit a decrease in Myc protein. The result of this decrease is the spontaneous differentiation into cells that resemble retinoic acid-treated F9 cells as judged by plasminogen activator assays. In contrast, when F9 cells are transfected with a plasmid expressing Myc under control of the SV40 early promoter, resulting cell clones are resistant to differentiation by retinoic acid as shown by the lack of induction of plasminogen activator. These results suggest that down-regulation of Myc is sufficient and necessary for F9 cell differentiation.