Hepatic dopamine sulfotransferases in untreated rats and in rats subjected to endocrine or hypertension-related treatments.

Mark Lucarelli // Publications // Nov 01 1988

PubMed ID: 3192164

Author(s): Singer SS, Palmert MR, Redman MD, Leahy DM, Feeser TC, Lucarelli MJ, Volkwein LS, Bruns M. Hepatic dopamine sulfotransferases in untreated rats and in rats subjected to endocrine or hypertension-related treatments. Hepatology. 1988 Nov-Dec;8(6):1511-20. PMID 3192164

Journal: Hepatology (Baltimore, Md.), Volume 8, Issue 6,

Here we describe the dopamine sulfotransferase activity of rat liver cytosol. With cytosol, 3′-phosphoadenosine-5′-phosphosulfate and dopamine Km values were 17.2 +/- 4.1 and 22.4 +/- 3.5 microM. Females possessed 23 to 37% of dopamine sulfotransferase levels, per gm liver, in males. DEAE-Sephadex A-50 chromatography resolved dopamine sulfotransferase activity to dopamine sulfotransferase I and dopamine sulfotransferase II. Dopamine sulfotransferase II comprised 79 +/- 10 or 61 +/- 18% of dopamine sulfotransferase in males or females in routine assays. 4-Methoxytyramine gave 609 or 179% of mean dopamine sulfotransferase activity with dopamine sulfotransferase I or II. Dopamine and 3-methoxytyramine were comparable substrates. Epinephrine was less effective. Mn++, Cd++, Zn++, Na+ and K+ inhibited dopamine sulfotransferase II. Mg++ activated it. Dopamine sulfotransferase II from males was purified 184 +/- 64-fold. Its Km values for 3′-phosphoadenosine-5′-phosphosulfate and dopamine were 12.7 +/- 1.5 and 47.5 +/- 6.7 microM, respectively. Its dopamine sulfotransferase mechanism was sequential. The molecular weight of dopamine sulfotransferase II was 49,100 +/- 4,000 by Sephadex G-100 chromatography. Dopamine sulfotransferase II preferred phenol to catecholamines. Dopamine and 3,4-dihydroxybenzylamine were its best catecholamine substrates. Adrenalectomy or castration of males led to 35 or 45% mean decreases of dopamine sulfotransferase levels, indicating adrenal and gonadal participation in control of dopamine sulfotransferase production. Testosterone had no effect in either sex, whereas estradiol led to 40% mean decreases of dopamine sulfotransferase levels in males. This suggested a role for ovaries in dopamine sulfotransferase production, supported by 55 to 102% increased dopamine sulfotransferase levels after ovariectomy. Okamoto-hypertensive males or males given hypertensogenic doses of cortisol exhibited 37 or 48% mean increases of dopamine sulfotransferase levels per gm liver. Antihypertensive spironolactone or hydralazine led to 30% mean decreases of dopamine sulfotransferase levels. Altered dopamine sulfotransferase levels after all experimental manipulations were due mostly to changed dopamine sulfotransferase II content. Dopamine sulfotransferase II is compared to other reported enzymes that sulfate catecholamines.