Accuracy of a filter paper method for measuring glycosylated hemoglobin.

Cruickshanks Lab // Publications // Jan 01 1992

PubMed ID: 1737527

Author(s): Gay EC, Cruickshanks KJ, Chase HP, Klingensmith G, Hamman RF. Accuracy of a filter paper method for measuring glycosylated hemoglobin. Diabetes Care. 1992 Jan;15(1):108-10. PMID 1737527

Journal: Diabetes Care, Volume 15, Issue 1, Jan 1992

OBJECTIVE To compare glycosylated hemoglobin (GH) results obtained by filter paper fingerstick collection and mailed for assay by affinity chromatography with results from a venous sample assayed by ion-exchange chromatography (HbA1) in a local laboratory.

RESEARCH DESIGN AND METHODS Fifty-eight volunteer subjects with insulin-dependent diabetes mellitus (IDDM), aged 5-24 yr, included patients at a referral-based IDDM clinic and subjects in an ongoing research study. We obtained two blood samples from each subject. One was collected by fingerstick onto filter paper, the other by venipuncture into a vacutainer. We sent filter paper samples to the Diabetes Research Laboratory (Univ. of Missouri, Columbia, MO) for analysis. Vacutainer samples were sent to the Clinical Chemistry Department of the Clinical Laboratory, University of Colorado Health Sciences Center.

RESULTS Results were highly correlated (r = 0.89, P = 0.0001). Fifty-nine percent were classified identically when results were normalized to SD units and grouped to suggest levels of clinical concern.

CONCLUSIONS The filter paper method is a convenient, accurate measure of glycosylated hemoglobin in young people with IDDM. It should be considered a credible alternative research and clinical tool.