Effect of staurosporine on outflow facility in monkeys.

Kaufman Lab // Publications // Apr 01 1999

PubMed ID: 10102301

Author(s): Tian B, Gabelt BT, Kaufman PL. Effect of staurosporine on outflow facility in monkeys. Invest Ophthalmol Vis Sci. 1999 Apr;40(5):1009-11. PMID 10102301

Journal: Investigative Ophthalmology & Visual Science, Volume 40, Issue 5, Apr 1999

PURPOSE To determine the effect of the serine-threonine kinase inhibitor staurosporine on outflow facility in living monkeys.

METHODS Total outflow facility was determined by two-level constant pressure perfusion of the anterior chamber bilaterally before and after intracameral infusion of staurosporine or vehicle in opposite eyes.

RESULTS Intracameral staurosporine dose-dependently doubled outflow facility, with 0.1 microM, 1 microM, and 10 microM being subthreshold, effective, and maximal doses, respectively. At 50 microM, intracameral staurosporine was less effective than 10 microM on facility and induced corneal toxicity.

CONCLUSIONS The broad-spectrum protein kinase inhibitor staurosporine increases outflow facility in living monkeys, perhaps by affecting the trabecular meshwork cytoskeleton.