Requirement for Dlgh-1 in planar cell polarity and skeletogenesis during vertebrate development.

Anne Griep // Publications // Jan 01 2013

PubMed ID: 23349879

Author(s): Rivera C, Simonson SJ, Yamben IF, Shatadal S, Nguyen MM, Beurg M, Lambert PF, Griep AE. Requirement for Dlgh-1 in planar cell polarity and skeletogenesis during vertebrate development. PLoS One. 2013;8(1):e54410. doi: 10.1371/journal.pone.0054410. Epub 2013 Jan 22. PMID 23349879

Journal: Plo S One, Volume 8, Issue 1, 2013

The development of specialized organs is tightly linked to the regulation of cell growth, orientation, migration and adhesion during embryogenesis. In addition, the directed movements of cells and their orientation within the plane of a tissue, termed planar cell polarity (PCP), appear to be crucial for the proper formation of the body plan. In Drosophila embryogenesis, Discs large (dlg) plays a critical role in apical-basal cell polarity, cell adhesion and cell proliferation. Craniofacial defects in mice carrying an insertional mutation in Dlgh-1 suggest that Dlgh-1 is required for vertebrate development. To determine what roles Dlgh-1 plays in vertebrate development, we generated mice carrying a null mutation in Dlgh-1. We found that deletion of Dlgh-1 caused open eyelids, open neural tube, and misorientation of cochlear hair cell stereociliary bundles, indicative of defects in planar cell polarity (PCP). Deletion of Dlgh-1 also caused skeletal defects throughout the embryo. These findings identify novel roles for Dlgh-1 in vertebrates that differ from its well-characterized roles in invertebrates and suggest that the Dlgh-1 null mouse may be a useful animal model to study certain human congenital birth defects.