Visualization of retinal vascular structure and perfusion with a nonconfocal adaptive optics scanning light ophthalmoscope.

Alfredo Dubra // Publications // Mar 01 2014

PubMed ID: 24690655

Author(s): Sulai YN, Scoles D, Harvey Z, Dubra A. Visualization of retinal vascular structure and perfusion with a nonconfocal adaptive optics scanning light ophthalmoscope. J Opt Soc Am A Opt Image Sci Vis. 2014 Mar 1;31(3):569-79. doi: 10.1364/JOSAA.31.000569. PMID 24690655

Journal: Journal Of The Optical Society Of America. A, Optics, Image Science, And Vision, Volume 31, Issue 3, Mar 2014

Imaging of the retinal vascular structure and perfusion was explored by confocal illumination and nonconfocal detection in an adaptive optics scanning light ophthalmoscope (AOSLO), as an extension of the work by Chui et al. [Biomed. Opt. Express 3, 2537 (2012)]. Five different detection schemes were evaluated at multiple retinal locations: circular mask, annular mask, circular mask with filament, knife-edge, and split-detector. Given the superior image contrast in the reflectance and perfusion maps, the split-detection method was further tested using pupil apodization, polarized detection, and four different wavelengths. None of these variations provided noticeable contrast improvement. The noninvasive visualization of capillary flow and structure provided by AOSLO split-detection shows great promise for studying ocular and systemic conditions that affect the retinal vasculature.