Heme degradation upon production of endogenous hydrogen peroxide via interaction of hemoglobin with sodium dodecyl sulfate.

Publications // Sheibani Lab // Apr 05 2014

PubMed ID: 24658005

Author(s): Salehi N, Moosavi-Movahedi AA, Fotouhi L, Yousefinejad S, Shourian M, Hosseinzadeh R, Sheibani N, Habibi-Rezaei M. Heme degradation upon production of endogenous hydrogen peroxide via interaction of hemoglobin with sodium dodecyl sulfate. J Photochem Photobiol B. 2014 Apr 5;133:11-7. doi: 10.1016/j.jphotobiol.2014.02.014. Epub 2014 Mar 6. PMID 24658005

Journal: Journal Of Photochemistry And Photobiology. B, Biology, Volume 133, Apr 2014

In this study the hemoglobin heme degradation upon interaction with sodium dodecyl sulfate (SDS) was investigated using UV-vis and fluorescence spectroscopy, multivariate curve resolution analysis, and chemiluminescence method. Our results showed that heme degradation occurred during interaction of hemoglobin with SDS producing three fluorescent components. We showed that the hydrogen peroxide, produced during this interaction, caused heme degradation. In addition, the endogenous hydrogen peroxide was more effective in hemoglobin heme degradation compared to exogenously added hydrogen peroxide. The endogenous form of hydrogen peroxide altered oxyHb to aquamethemoglobin and hemichrome at low concentration. In contrast, the exogenous hydrogen peroxide lacked this ability under same conditions.

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