Non-invasive assessment of human cone photoreceptor function.

Alfredo Dubra // Publications // Nov 01 2017

PubMed ID: 29188106

Author(s): Cooper RF, Tuten WS, Dubra A, Brainard DH, Morgan JIW. Non-invasive assessment of human cone photoreceptor function. Biomed Opt Express. 2017 Oct 19;8(11):5098-5112. doi: 10.1364/BOE.8.005098. eCollection 2017 Nov 1. Erratum in: Biomed Opt Express. 2018 Mar 22;9(4):1842. PMID 29188106

Journal: Biomedical Optics Express, Volume 8, Issue 11, Nov 2017

Vision begins when light isomerizes the photopigments within photoreceptors. Noninvasive cellular-scale observation of the structure of the human photoreceptor mosaic is made possible through the use of adaptive optics (AO) enhanced ophthalmoscopes, but establishing noninvasive objective measures of photoreceptor function on a similar scale has been more difficult. AO ophthalmoscope images acquired with near-infrared light show that individual cone photoreceptor reflectance can change in response to a visible stimulus. Here we show that the intrinsic response depends on stimulus wavelength and intensity, and that its action spectrum is well-matched to the spectral sensitivity of cone-mediated vision. Our results demonstrate that the cone reflectance response is mediated by photoisomerization, thus making it a direct measure of photoreceptor function.