Characterization of human uveal melanoma cells by phosphorus 31 nuclear magnetic resonance spectroscopy.

Daniel Albert // Publications // Jul 15 1985

PubMed ID: 4014378

Author(s): Kolodny NH, Albert DM, Epstein J, Ruzzo M, Sprengnether M. Characterization of human uveal melanoma cells by phosphorus 31 nuclear magnetic resonance spectroscopy. Am J Ophthalmol. 1985 Jul 15;100(1):38-44. PMID 4014378

Journal: American Journal Of Ophthalmology, Volume 100, Issue 1, Jul 1985

We performed experiments to determine the potential usefulness of nuclear magnetic resonance spectra in the diagnosis and follow-up of ocular melanoma. High-resolution phosphorus 31 nuclear magnetic resonance spectra at 109.3 MHz were obtained for human uveal melanoma, Greene hamster melanoma, and normal human diploid fibroblast cells. Phosphate metabolites were identified and their concentrations were shown to vary among the different cell lines. Uveal melanoma cells contain unusually high concentrations of the phospholipid metabolite phosphorylcholine and the phosphodiesters glycerol 3-phosphoryl choline and glycerol 3-phosphoryl ethanolamine. Baseline data are thus provided for studies of the effect of various treatment modalities on uveal melanoma. These initial results suggest that the data provided by high-resolution phosphorus 31 nuclear magnetic resonance spectra can provide useful diagnostic and follow-up data with respect to ocular melanoma.