A perfusion system developed for 31P NMR study of melanoma cells at tissue-like density.

Daniel Albert // Publications // Apr 01 1989

PubMed ID: 2755337

Author(s): Minichiello MM, Albert DM, Kolodny NH, Lee MS, Craft JL. A perfusion system developed for 31P NMR study of melanoma cells at tissue-like density. Magn Reson Med. 1989 Apr;10(1):96-107. PMID 2755337

Journal: Magnetic Resonance In Medicine, Volume 10, Issue 1, Apr 1989

A perfusion culture system has been developed for 31P NMR study of human uveal melanoma metabolism by adapting the Vitafiber I cartridge system (Amicon). 31P NMR spectra collected weekly during periods of up to 10 weeks demonstrated increasing levels of phosphorus metabolites as the anchorage-dependent cells grew to tissue-like density.