Interferon-alpha and interferon-gamma induced modulation of proteins in human corneal fibroblasts.

Brandt Lab // Publications // Aug 01 1993

PubMed ID: 8228389

Author(s): Balish MJ, Abrams ME, Chandler JW, Brandt CR. Interferon-alpha and interferon-gamma induced modulation of proteins in human corneal fibroblasts. J Interferon Res. 1993 Aug;13(4):289-94. PMID 8228389

Journal: Journal Of Interferon Research, Volume 13, Issue 4, Aug 1993

Little is known about the effects of interferon (IFN) on cell function in the eye. We have analyzed the effect of INF-alpha and IFN-gamma on the expression of proteins in cultured human corneal fibroblasts. Treatment with IFN-alpha increased the synthesis of proteins of 84, 76, 52, and 28 kD and decreased the synthesis of a 72-kD protein. Treatment with IFN-gamma increased the synthesis of proteins of 83, 66, 64, 54, and 47 kD. The effect of IFN-alpha and IFN-gamma were first detected at 5-9 h and 9 h, respectively, after the addition of the IFNs and were maximal at 17 and 24 h, respectively. Most of the changes were seen at doses of 1 x 10(1) to 1 x 10(2) U/ml of IFN-alpha or IFN-gamma and were maximal at 1 x 10(2) to 1 x 10(3) U/ml. Thus, each IFN induced distinct proteins based on apparent molecular weight and isoelectric point. These results show that IFN-alpha and IFN-gamma affect the synthesis of small groups of distinct proteins in human corneal fibroblasts.