IFN-alpha induces MxA gene expression in cultured human corneal fibroblasts.

Brandt Lab // Publications // Feb 01 1995

PubMed ID: 7540146

Author(s): Abrams ME, Balish MJ, Brandt CR. IFN-alpha induces MxA gene expression in cultured human corneal fibroblasts. Exp Eye Res. 1995 Feb;60(2):137-42.

Journal: Experimental Eye Research, Volume 60, Issue 2, Feb 1995

Treatment of certain human cells with Interferon-alpha (IFN-alpha) induces the synthesis of a 76 kDa protein designated MxA that is involved in resistance to viral infection. We have used a specific cDNA clone and monoclonal Ab to show that MxA is induced in IFN-alpha treated human corneal fibroblast cultures. Mx RNA was increased 23-fold and 45-fold after 5 and 9 h of IFN-alpha treatment, respectively. The MxA protein was detectable by immunoblotting at 5 hr after IFN treatment and peaked at 17 hr. Concentrations of IFN-alpha as low as 1 U/ml induced detectable amounts of MxA, and expression was maximal at 1 x 10(3) U ml-1. These results confirm that MxA synthesis is induced in human corneal fibroblasts treated with IFN-alpha.