Author(s): Sachsenmeier KF, Sheibani N, Schlosser SJ, Allen-Hoffmann BL. Transforming growth factor-beta 1 inhibits nucleosomal fragmentation in human keratinocytes following loss of adhesion. J Biol Chem. 1996 Jan 5;271(1):5-8.
Journal: The Journal Of Biological Chemistry, Volume 271, Issue 1, Jan 1996
We have investigated the role of transforming growth factor-beta 1 (TGF-beta 1) in suspension-induced programmed cell death of cultured human keratinocytes. Suspension of keratinocytes in semisolid medium induces TGF-beta 1 mRNA levels and synthesis of bioactive TGF-beta 1 protein. Concomitant with the suspension-induced increase in secreted TGF-beta 1 levels, steady state mRNA levels for c-myc are decreased. Both exogenously added and endogenously produced TGF-beta 1 attenuate suspension-induced nucleosomal fragmentation in keratinocytes. We propose that TGF-beta 1 may function to protect keratinocytes from DNA fragmentation following loss of cell-substratum and/or cell-cell contact. Taken together, our findings suggest that loss of cell-substratum and/or cell-cell adhesion is an important component of an apoptotic signal transduction cascade regulated by TGF-beta 1 in normal human stratified squamous epithelia.