Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy.

Alfredo Dubra // Publications // Dec 17 2010

PubMed ID: 21326644

Author(s): Hunter JJ, Masella B, Dubra A, Sharma R, Yin L, Merigan WH, Palczewska G, Palczewski K, Williams DR. Images of photoreceptors in living primate eyes using adaptive optics two-photon ophthalmoscopy. Biomed Opt Express. 2010 Dec 17;2(1):139-48. doi: 10.1364/BOE.2.000139. PMID 21326644

Journal: Biomedical Optics Express, Volume 2, Issue 1, Dec 2010

In vivo two-photon imaging through the pupil of the primate eye has the potential to become a useful tool for functional imaging of the retina. Two-photon excited fluorescence images of the macaque cone mosaic were obtained using a fluorescence adaptive optics scanning laser ophthalmoscope, overcoming the challenges of a low numerical aperture, imperfect optics of the eye, high required light levels, and eye motion. Although the specific fluorophores are as yet unknown, strong in vivo intrinsic fluorescence allowed images of the cone mosaic. Imaging intact ex vivo retina revealed that the strongest two-photon excited fluorescence signal comes from the cone inner segments. The fluorescence response increased following light stimulation, which could provide a functional measure of the effects of light on photoreceptors.