Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II.

PubMed ID: 22829053

Author(s): Fallah-Bagheri A, Saboury AA, Ma’mani L, Taghizadeh M, Khodarahmi R, Ranjbar S, Bohlooli M, Shafiee A, Foroumadi A, Sheibani N, Moosavi-Movahedi AA. Effects of silica nanoparticle supported ionic liquid as additive on thermal reversibility of human carbonic anhydrase II. Int J Biol Macromol. 2012 Dec;51(5):933-8. doi: 10.1016/j.ijbiomac.2012.07.023. Epub 2012 Jul 22. PMID 22829053

Journal: International Journal Of Biological Macromolecules, Volume 51, Issue 5, Dec 2012

Silica nanoparticle supported imidazolium ionic liquid [SNImIL] was synthesized and utilized as a biocompatible additive for studying the thermal reversibility of human carbonic anhydrase II (HCA II). For this purpose, we prepared additive by modification of nanoparticles through the grafting of ionic liquids on the surface of nanoparticles (SNImIL). The SNImIL were fully characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and thermo gravimetric analysis. The characterization of HCA II was investigated by various techniques including UV-vis and ANS fluorescence spectrophotometry, differential scanning calorimetry, and docking study. SNImIL induced disaggregation, enhanced protein stability and increased thermal reversibility of HCA II by up to 42% at pH 7.75.