Denaturation and intermediates study of two sturgeon hemoglobins by n-dodecyl trimethylammonium bromide.

Publications // Sheibani Lab // Feb 01 2013

PubMed ID: 23142155

Author(s): Ariaeenejad S, Habibi-Rezaei M, Kavousi K, Jamili S, Fatemi MR, Hong J, Poursasan N, Sheibani N, Moosavi-Movahedi AA. Denaturation and intermediates study of two sturgeon hemoglobins by n-dodecyl trimethylammonium bromide. Int J Biol Macromol. 2013 Feb;53:107-13. doi: 10.1016/j.ijbiomac.2012.10.015. Epub 2012 Nov 9. PMID 23142155

Journal: International Journal Of Biological Macromolecules, Volume 53, Feb 2013

Varieties of hemoglobin (Hb) forms exist in fish, which are usually well adapted to the different ecological conditions or various habitats. In the current study, Hbs from two Sturgeon species of the Southern Caspian Sea Basin were purified and studied upon interaction with n-dodecyl trimethylammonium bromide (DTAB; as a cationic surfactant) by various methods including UV-visible absorption, dynamic light scattering (DLS), and ANS fluorescence spectrophotometry. The chemometric analysis of Hbs was investigated upon interaction with DTAB under titration, using UV-visible absorption spectra. The chemometric resolution techniques were used to determine the number of the components and mole fraction of the oxidized Hbs. These results provided the evidence for the existence of three different molecular components including native (N), intermediate (I) and denatured (D) in sturgeon Hbs. According to the distribution of intermediates, which were broadened in a range of DTAB concentration, the aggregation states, DLS experiments, and thermal stability (T(m) obtained by differential scanning calorimetry (DSC)), the Acipenser stellatus Hb was more stable compared to Acipenser persicus Hb. These results demonstrate a significant relationship between the stability of fish Hbs and the habitat depth requirements.

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