Simultaneous Fluorescein Angiography and Spectral Domain Optical Coherence Tomography Correlate Retinal Thickness Changes to Vascular Abnormalities in an In Vivo Mouse Model of Retinopathy of Prematurity.

Olachi Mezu-Ndubuisi // Publications // Jan 01 2017

PubMed ID: 28573047

Author(s): Mezu-Ndubuisi OJ, Taylor LK, Schoephoerster JA. Simultaneous fluorescein angiography and spectral domain optical coherence tomography correlate retinal thickness changes to vascular abnormalities in an in vivo mouse model of retinopathy of prematurity. J Ophthalmol. 2017;2017:9620876. doi: 10.1155/2017/9620876. Epub 2017 May 10. PMID 28573047

Journal: Journal Of Ophthalmology, Volume 2017, 2017

BACKGROUND Retinopathy of prematurity (ROP) is a condition of abnormal retinal vascular development (RVD) in premature infants. Fluorescein angiography (FA) has depicted phases (early, mid, late, and mature) of RVD in oxygen-induced retinopathy (OIR) mice. We sought to establish the relationship between retinal structural and vascular changes using simultaneous FA and spectral domain optical coherence tomography (SD-OCT).

METHOD 63 mice were exposed to 77% oxygen at postnatal day 7 (P7) for 5 days, while 63 mice remained in room air (RA). Total retinal thickness (TRT), inner retinal thickness (IRT), and outer retinal thickness (ORT) were calculated at early (P19), mid (P24), late (P32), and mature (P47) phases of RVD.

RESULTS TRT was reduced in OIR (162.66 ± 17.75 μm, n = 13) compared to RA mice at P19 (197.57 ± 3.49 μm, n = 14), P24, P32, and P49 (P 0.05). IRT was reduced in OIR (71.60 ± 17.14 μm) compared to RA (103.07 ± 3.47 μm) mice at P19 and all ages (P < 0.0001).

CONCLUSION We have shown the spatial and temporal relationship between retinal structure and vascular development in OIR. Significant inner retinal thinning in OIR mice persisted despite revascularization of the capillary network; further studies will elucidate its functional implications in ROP.