Detection of Guanine and Adenine Using an Aminated Reduced Graphene Oxide Functional Membrane-Modified Glassy Carbon Electrode.

Publications // Sheibani Lab // Jul 18 2017

PubMed ID: 28718793

Author(s): Li D, Yang XL, Xiao BL, Geng FY, Hong J, Sheibani N, Moosavi-Movahedi AA. Detection of guanine and adenine using an aminated reduced graphene oxide functional membrane-modified glassy carbon electrode. Sensors (Basel). 2017 Jul 18;17(7). pii: E1652. doi: 10.3390/s17071652. PMID 28718793

Journal: Sensors (Basel, Switzerland), Volume 17, Issue 7, Jul 2017

A new electrochemical sensor based on a Nafion, aminated reduced graphene oxide and chitosan functional membrane-modified glassy carbon electrode was proposed for the simultaneous detection of adenine and guanine. Fourier transform-infrared spectrometry (FTIR), transmission electron microscopy (TEM), and electrochemical methods were utilized for the additional characterization of the membrane materials. The prepared electrode was utilized for the detection of guanine (G) and adenine (A). The anodic peak currents to G and A were linear in the concentrations ranging from 0.1 to 120 μM and 0.2 to 110 μM, respectively. The detection limits were found to be 0.1 μM and 0.2 μM, respectively. Moreover, the modified electrode could also be used to determine G and A in calf thymus DNA.