Author(s): Akondi V, Dubra A. Multi-layer Shack-Hartmann wavefront sensing in the point source regime. Biomed Opt Express. 2020 Dec 16;12(1):409-432. doi: 10.1364/BOE.411189. eCollection 2021 Jan 1. PMID 33520390
Journal: Biomedical Optics Express, Volume 12, Issue 1, Jan 2021
The Shack-Hartmann wavefront sensor (SHWS) is often operated under the assumption that the sensed light can be described by a single wavefront. In biological tissues and other multi-layered samples, secondary wavefronts from axially and/or transversely displaced regions can lead to artifactual aberrations. Here, we evaluate these artifactual aberrations in a simulated ophthalmic SHWS by modeling the beacons that would be generated by a two-layer retina in human and mouse eyes. Then, we propose formulae for calculating a minimum SHWS centroid integration area to mitigate these aberrations by an order of magnitude, potentially benefiting SHWS-based metrology and adaptive optics systems such as those used for retinal imaging and microscopy.