Phalloidin inhibits epinephrine’s and cytochalasin B’s facilitation of aqueous outflow.

Kaufman Lab // Publications // Dec 01 1994

PubMed ID: 7993218

Author(s): Robinson JC, Kaufman PL. Phalloidin inhibits epinephrine’s and cytochalasin B’s facilitation of aqueous outflow. Arch Ophthalmol. 1994 Dec;112(12):1610-3. PMID 7993218

Journal: Archives Of Ophthalmology (Chicago, Ill. : 1960), Volume 112, Issue 12, Dec 1994

OBJECTIVE To determine whether phalloidin, a fungal peptide that inhibits actin filament depolymerization, could inhibit the ability of cytochalasin B and epinephrine to increase the facility of aqueous outflow in the eyes of living cynomolgus monkeys.

METHODS Outflow facility was determined by two-level constant-pressure perfusion of the anterior chamber. After measurement of baseline facility in both eyes, one eye of each animal received intracameral phalloidin (1.3, 13, or 130 mumol/L); the opposite eye received vehicle. Both eyes then received either epinephrine (0.3 mmol/L) or cytochalasin B (0.2 mmol/L), and facility was again measured.

RESULTS Cytochalasin B and epinephrine increased facility by 120% to 190% and 100% to 180%, respectively (uncorrected for 15% resistance washout caused by perfusion itself). Phalloidin itself (13 or 130 mumol/L) did not affect facility, but it inhibited up to 50% of the facility-increasing effect of cytochalasin B and epinephrine.

CONCLUSIONS We conclude that (1) the aqueous humor outflow facilitating effects of cytochalasin B or epinephrine depend in some manner on depolymerization of actin filaments within trabecular meshwork cells, and (2) actin filaments may help regulate aqueous outflow.